Chemical characterization and in vitro antioxidant activity of hydroethanolic leaf extracts of Pontederia crassipes Mart
DOI:
https://doi.org/10.5281/zenodo.17916301Keywords:
anthraquinones, aquatic plant, flavonoids, phenolsAbstract
Introduction: Pontederia crassipes Mart. is an invasive, damaging, and transformative aquatic plant found in artificial freshwater lagoons in Ciego de Ávila, Cuba. Despite studies demonstrating the benefits it provides to humans, there are no references to its use for a specific purpose in Cuba. Objective: to determine the ideal temperature for obtaining hydroethanolic leaf extracts of P. crassipes (emergent macrophyte), in vitro, with higher yield of phenols compounds, flavonoids, anthraquinones and antioxidant activity. Method: the extracts were obtained at different temperatures (27°C, 40°C, 60°C, and 80°C). Ethanol at 90% (v:v) was used as solvent for a solid-liquid ratio of 1:30 (m:v). Results: leaf extracts did not show significant differences in yield (p= 0.5903) and anthraquinone concentration (p= 0.24). The highest concentration of phenols was obtained in extracts at 60°C (10.04 mg g-1), with no significant differences from the other temperatures. The highest concentration of flavonoids and antioxidant activity was obtained in extracts at 60°C (7.40 mg g-1 and 118.57 µg mL-1) and 80°C (7.38 mg g-1 and 117.12 µg mL-1), respectively. These results differ from those obtained in P. crassipes, with grown in La Turbina lagoon. Conclusion: 60°C was selected as the ideal temperature for obtaining hydroethanolic leaf extracts of P. crassipes. P. crassipes extracts have potential as antioxidants for the pharmaceutical, agricultural and cosmetic industries. Systematic studies are needed using P. crassipes specimens obtained from various ecosystems.
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