Detection of Ehrlichia canis using the Buffy coat method in dogs, ZERVET clinic, San Marcos, Nicaragua
DOI:
https://doi.org/10.5281/zenodo.17885811Keywords:
buffy coat, platelet concentrate, whole blood, zero prevalenceAbstract
Introduction: Early diagnosis of Ehrlichia canis in dogs is essential for the management of canine ehrlichiosis, a serious disease affecting canines in tropical and subtropical regions such as Nicaragua. Although seroprevalence studies have been conducted using serological methods such as ELISA, there is little research on the effectiveness of the Buffy coat method. Objective: To diagnose the presence of Ehrlichia canis using the Buffy coat method in dogs treated at the ZERVET veterinary clinic, San Marcos, Nicaragua. Objective: Diagnosis of Ehrlichia canis infection using the Buffy coat method in dogs attended at the ZERVET Veterinary Clinic, San Marcos, Nicaragua. Method: A descriptive cross-sectional study was conducted, analyzing blood samples by microscopic observation. Criteria for sample selection were established, and staining techniques were used to visualize the microorganisms. An analysis of different updated research related to this topic was performed. Results: The prevalence of Ehrlichia canis reached 52.5 %, and its complete analysis revealed the sensitivity and specificity of the Buffy Coat method compared to other diagnostic techniques. It was shown that mixed-breed dogs are more predisposed to ehrlichiosis infection; adult dogs are more susceptible to this infection than month-old puppies; there is no selectivity on the part of the microorganism with respect to sex; urban and rural dogs are equally vulnerable to ehrlichiosis, because the transmitting tick adapts to different environments.
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Alvarado, E., Flores, V. y Sempertegui, C. (2024). Incidencia de Ehrlichia canis en caninos mediante la tinción de GIEMSA del distrito II del Municipio de Cobija . Libro DICyT Área Ciencias Biológicas y Naturales, 1(1), 15-15. https://doi.org/https://dx.doi.org/10.37885/221211416
Aragón López, C., Luna Nevárez, P., Ortiz Encinas, V., Corona, L. J., Cantu Soto, E. y Granados, R. J. (2022). Detección molecular de Ehrlichia canis, Anaplasma platys y Rickettsia rickettsii en caninos domésticos del municipio de Cajeme, Sonora, México. SCIELO, 11. https://doi.org/10.21929/abavet2021.45
Beristain Ruiz, D. M., Garza Hernandez, J. A., Figueroa Milan , J. V., Lira Amaya, J. J., Quesada Casasola, A., Ordoñez López, S., ... Rodriguez Alarcón, C. A. (2022). Possible Association between Selected Tick Borne Pathogen Prevalence and Rhipicephalus sanguineus sensu lato infestation in dogs from Juarez City (Chihuahua), Northwest Mexico–US Border. Pathogens, 11(5), 552. https://doi.org/https://doi.org/10.3390/pathogens11050552
Carbajal, A. y Vilela, J. (2024). Frecuencia y factores asociados al diagnóstico deEhrlichia canis yAnaplasma spp.en perros. Rev Mex CiencPecu, 15(3), 13. https://doi.org/https://doi.org/10.22319/rmcp.v15i3.6604
Delgado, N., Saucedo, A., Martínez, L., Herrera, J. D. y Hernandez, J. (2024). Ehrlichiosis canina en Pungarabato, Guerrero, México. FCV-LUZ, XXXIV, 4. https://doi.org/https://doi.org/10.52973/rcfcv-e34492
Espino Solis, G. P., Flores Lira, E. A., Barreras Serrano, A., García Reynoso, I. C., De la Mora Covarrubias, A., Jiménz Vega, F. y Escárcega Ávila, A. (2023). Clinical and pathological factors associated with Erlichia canis in companion dogs. The Journal of infection in Developing Countries, 17(11), 1598-1605. https://doi.org/Https://doi.org/10.3855/jidc.17961
Ferrolho, J., Antunes, S., Vilhena, H., S, A., De Sousa, R. S., S, F. G., ... Domingos, A. (2025). The complexities of canine monocytic ehrlichiosis: Insights into Ehrlichia canis and its vector Rhipicephalus sanguineus. Microbiology Research,, 16(4). https://doi.org/https://doi.org/10.3390/microbiolres16040085
Guendulain, C., Tamiozzo, P., Anbal, B. y Caffaratti, M. (2021). Detección molecular de Ehrlichia canis en perros de diferentes localidades de la provincia de Córdoba, Argentina. Ab Intus FAV-UNRC, 7(4), 44, 45. https://www.ayv.unrc.edu.ar/ojs/index.php/Ab_Intus/article/view/31/184
Mansilla, S. L., Delgado, M. B., Rossner M, V., Cainzos, R. P., Merino, L. A. y Koscinczuk, P. (2023). Alteraciones hematológicas en perros (Canis lupus familiaris) diagnosticados con Ehrlichia spp. por PCR, en clínicas veterinarias del Nordeste Argentino. REVISTA VETERINARIA, 34(2), 91-95. https://doi.org/https://doi.org/10.30972/vet.3427049
Medina, H. (2024). Presencia de Ehrlichia en canes atendidos en la clínica “Mundo Animal”. Babahoyo Por ti UTB, 39. https://doi.org/http://dspace.utb.edu.ec/handle/49000/16319
Merino, O., Badillo, V., Loredo, J., Barrios, H. y Carvajal, V. (2021). Detección molecular de Ehrlichia canis y Anaplasma phagocytophilum y alteraciones hematológicas de perros infectados. Abanico Veterinario, 11, 16. https://doi.org/https://doi.org/10.21929/abavet2021.29
Montero, C. (2024). Identificación de Ehrlichia canis en perros que presentan antecedentes de ectoparásitosis atendidos en el centro veterinario ‘Dr. JEFF’ del Cantón Ventanas, Provincia de Los Ríos. DSpace UTB, 23. https://doi.org/http://dspace.utb.edu.ec/handle/49000/17361
Richarson, S., Mainville, C., Arguello Marin, A., Walley , D., Burton, W., Breitschwerdt, E. y Qurollo, B. (2023). Un inmunoensayo de segunda generación en el punto de atención proporcionó una mejor detección de anticuerpos contra Anaplasma y Ehrlichia en perros PCR positivos infectados naturalmente con especies de Anaplasma o Ehrlichia. Revista de investigación diagnostica veterinaria, 35(4). https://doi.org/https://doi.org/10.1177/10406387231172723
Rodriguez Alarcón, C. A., Beristain Ruiz, D. M., Olivarez Muñoz, A., Quezada Casasola, A., Pérez Casio, F., Alvarez Martinez, J. A. y Cera Hurtado, O. S. (2020). Demonstrating the presence of Ehrlichia canis DNA from different tissues of dogs with suspected subclinical ehrlichiosis. Parasites & Vectors, 13. https://doi.org/https://doi.org/10.1186/s13071-020-04363-0
Selim, A. (2021). Seroprevalencia y factores de riesgo asociados para patógenos transmitidos por vectores en perros de Egipto. Parasites vector, 14(175). https://doi.org/https://doi.org/10.1186/s13071-021-04670-0
Vasquez Borja, S., Aguilar Galvez, F. L., Guerrero López, A. E., Mocha Cuenca, B. J., Baylon Cuva, M. V. y Sanchez Prado, R. (2024). Detección molecular con la técnica PCRen un solo paso para Erlichia canis en perros del canton Ponce Enriquez. Ecuador. Ciencia latina revista cientifica multidisciplinar, 8(2). https://doi.org/http.//doi.org/10.37811/cl_rcm.v812.11234
Veterinaria, M. M. (25 de Abril de 2025). Ehrlichiosis, anaplasmosis e infecciones relacionadas en animales https://www.merckvetmanual.com/es-us/enfermedades-generalizadas/enfermedades-rickettsiales/ehrlichiosis-anaplasmosis-e-infecciones-relacionadas-en-animales
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