Antioxidant potential of essential oils from the leaves of Mosiera bullata subsp bullata and their sub-products of hydrodistillation
DOI:
https://doi.org/10.5281/zenodo.20595002Keywords:
endemic species, hydrodistillation, wastewaterAbstract
Introduction: currently, there is a growing interest in the use of plant extracts and essential oils with antioxidant properties. Mosiera bullata (Britton & P. Wilson) Bisse subsp. bullata is a species endemic to Cuba, belonging to the Myrtaceae family. However, to date, studies on the antioxidant potential of its essential oils and the byproducts obtained during the hydrodistillation process are limited. Objectives: establish a methodology for obtaining the essential oils; determine the physicochemical characteristics of the essential oils; evaluate the in vitro antioxidant activity of the oils, hydrosol, and wastewater obtained by hydrodistillation. Method: a methodology for oil extraction was established. The effect of the following variables on extraction yield was evaluated: extraction time and plant material mass/water volume ratio. The physicochemical characteristics were determined. The antioxidant activity of the oils, hydrosol, and wastewater was evaluated using DPPH• and ABTS•+ radical scavenging assays. Results: the highest oil yield (1.57 %) was obtained with a 1:5 (w:v) ratio for 3 h. The following properties were obtained: density (0.8708 ± 0.0006 g mL -1), refractive index at 20 °C (1.4567 ± 0.0014), acid value (4.21 ± 0.4661 mg KOH g-1), and solubility in 97 %, 95 %, and 90 % ethanol (positive) and soluble in 70 % ethanol (soluble in 14 volumes). In the tests with the DPPH• and ABTS•+ radicals, wastewater showed greater antioxidant activity (IC50 57.54 ± 0.35 µg·mL-1; 214.50 ± 2.31 µg·mL-1) than oils (IC50 3228.8 ± 3.12 µg·mL-1; 7929.9 ± 5.11 µg·mL-1) respectively. No antioxidant activity was detected for the hydrosol obtained as a byproduct within the evaluated concentration range. Conclusion: A procedure was established for obtaining the oils with the specified ratio for 3 h. The physicochemical characteristics demonstrated the quality of the oils and are within the range of parameters described for plants of the Myrtaceae family. The wastewater showed strong antioxidant activity.
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